rabbit pan neuronal marker protein gene product  (Bio-Rad)

Journal: BMC Nephrology

Article Title: The importance of a multidisciplinary approach in two tricky cases: the perfect match for Fabry disease

doi: 10.1186/s12882-025-04009-2

Figure Lengend Snippet: Negative Gb3 immunofluorescence performed on renal sample of Patient 2. ( a ) Negative Gb3 (red); ( b ) Merge of negative Gb3 (red), positive collagen (green), and nuclei (blue). 50-µm sections were prepared using a freezing sliding microtome (HM550, Thermo Scientific, Waltham, MA, USA) to evaluate Gb3 deposits. The sections were immunostained overnight with a panel of primary antibodies, including mouse monoclonal anti-Gb3 (1:1000; TCI Chemicals, Portland, Oregon, USA), rabbit pan-neuronal marker protein gene product 9.5 (1:1000; AbD Serotec, Raleigh, NC, USA) and rabbit polyclonal anti-collagen IV (1:500; Novus Biologicals, Littleton, CO, USA). After washing, secondary antibodies were applied for an additional overnight incubation. Mouse cyanine dye fluorophores 3.18 (1:800; Jackson ImmunoResearch, West Grove, PA, USA) were used as secondary antibodies. Sections were viewed and analysed under a Nikon confocal microscopy (Eclipse Ti A1)

Article Snippet: The sections were immunostained overnight with a panel of primary antibodies, including mouse monoclonal anti-Gb3 (1:1000; TCI Chemicals, Portland, Oregon, USA), rabbit pan-neuronal marker protein gene product 9.5 (1:1000; AbD Serotec, Raleigh, NC, USA) and rabbit polyclonal anti-collagen IV (1:500; Novus Biologicals, Littleton, CO, USA).

Techniques: Immunofluorescence, Marker, Incubation, Confocal Microscopy